Download scientific diagram | Identification and characterization of a broadly neutralizing monoclonal antibody from Smg immunized mice. a, FACS showing the percentage of spike-specific B cells from mice immunized with Sfg or Smg at day 28 after the last immunization. b, The relative ratio of the percentage of S-specific B cells (CD3 − CD19 + S + ) vs. negative staining (without S) in the spleen of immunized mice or naïve mice (n=5). c, FACS analysis of m31A7 binding with 293T cells expressing S protein of WH01 and various variants. d, ELISA showing m31A7 binding to S1, S2, or RBD of the S protein. from publication: Impact of Glycosylation on SARS-CoV-2 Infection and Broadly Protective Vaccine Design | A major challenge to end the pandemic caused by SARS-CoV-2 is to develop a broadly protective vaccine. As the key immunogen, the spike protein is frequently mutated with conserved epitopes shielded by glycans. Here, we reveal that spike glycosylation has site-differential... | Vaccine Development, COVID-19 and Protection | ResearchGate, the professional network for scientists.